Villin Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Villin Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen(19)

Kat-Nr. : 16488-1-AP

Synonyme

VIL1, D2S1471, VIL, villin 1

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  WB analysis of mouse kidney using 16488-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 16488-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 16488-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 16488-1-AP (Villin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16488-1-AP

  IP result of anti-Villin (IP:16488-1-AP, 4ug; Detection:16488-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

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  IHC staining of mouse small intestine using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 16488-1-AP (Villin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16488-1-AP (Villin antibody at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 16488-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16488-1-AP (Villin antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®647-conjugated AffiniPure F(ab, CoraLite® Plus 488 PIGR antibody (CL488-22024, green), CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

• 

  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse small intestine using 16488-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using Villin antibody (16488-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of COLO 320 using 16488-1-AP

  Immunofluorescent analysis of COLO 320 cells using 16488-1-AP (Villin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 16488-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human Villin (16488-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, Maus-Kolongewebe, Mauslebergewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IF/ICC: COLO 320-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 12 publications below

Produktinformation

16488-1-AP bindet in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA Villin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Villin fusion protein Ag9610
• Vollständiger Name: villin 1
• Berechnetes Molekulargewicht: 827aa,93 kDa; 826aa,93 kDa
• Beobachtetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC017303
• Gene symbol: Villin
• Gene ID (NCBI): 7429
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma. VIL1 was recently identified as a novel biomarker predictive for postoperative recurrence and poorer prognosis of high serum AFP associated HCC.

Publikationen

Species	Application	Title
rat	IF	Sci Transl Med; Oral delivery of liquid mRNA therapeutics by an engineered capsule for treatment of preclinical intestinal disease; Authors - Xiangang Huang
human	IF	Proc Natl Acad Sci U S A; Organotypic models of type III interferon-mediated protection from Zika virus infections at the maternal-fetal interface.; Authors - Jacqueline Corry
mouse	IF	Clin Transl Med; NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.; Authors - Zhixin Liu
human	WB	mBio; ASB3 expression aggravates inflammatory bowel disease by targeting TRAF6 protein stability and affecting the intestinal microbiota; Authors - Mingyang Cheng
pig	IF	PLoS Pathog; Bile acids promote the caveolae-associated entry of swine acute diarrhea syndrome coronavirus in porcine intestinal enteroids.; Authors - Qi-Yue Yang
mouse	WB	Front Cell Dev Biol; Cathelicidin-WA Protects Against LPS-Induced Gut Damage Through Enhancing Survival and Function of Intestinal Stem Cells.; Authors - Sisi Wang