VIP Polyklonaler Antikörper

VIP Polyklonal Antikörper für IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 16233-1-AP

Synonyme

Intestinal peptide PHM-27, Intestinal peptide PHV-42, VIP peptides

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  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 16233-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 16233-1-AP (VIP antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 16233-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using VIP antibody (16233-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HT-29 using 16233-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using VIP antibody (16233-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Dünndarmgewebe, Maushirngewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: HT-29-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

16233-1-AP bindet in WB, IHC, IF/ICC, IF-P, ELISA VIP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VIP fusion protein Ag8933
• Vollständiger Name: vasoactive intestinal peptide
• Berechnetes Molekulargewicht: 169 aa, 19 kDa
• GenBank-Zugangsnummer: BC009794
• Gene symbol: VIP
• Gene ID (NCBI): 7432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vasoactive intestinal peptide (VIP), a short peptide containing 28 amino acids belonging to the secretin-glucagon family, is initially isolated from the gastrointestinal tract as a potent vasodilator peptide. VIP was initially identified in normal nervous tissue and neurons and was subsequently recognized as a neurotransmitter widely distributed in various tissues. The wide distribution of VIP determines its involvement in a range of biological activities, such as gut motility, hormonal regulation, circadian rhythms, immune responses, and carcinogenesis. The general physiologic effects of VIP include vasodilation, anti-inflammatory actions, cell proliferation, hormonal secretion, regulation of gastric motility, and smooth muscle relaxation; therefore, VIP has emerged as a promising drug candidate for the treatment of several diseases.

Publikationen

Species	Application	Title
mouse	IHC	Immun Inflamm Dis; Vasoactive intestinal peptide exerts therapeutic action by regulating PTEN in a model of Sjögren's disease; Authors - Yixi Li
mouse	IF	Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
mouse	IF	Cell Rep; PMID: 31509750; Authors - Jing Xue
rat	WB	Biomolecules; Postweaning Development Influences Endogenous VPAC1 Modulation of LTP Induced by Theta-Burst Stimulation: A Link to Maturation of the Hippocampal GABAergic System; Authors - Marta Gil
rat	WB	J Neurosci Res; Mismatch novelty exploration training shifts VPAC1 receptor-mediated modulation of hippocampal synaptic plasticity by endogenous VIP in male rats; Authors - Fatima Aidil-Carvalho
mouse		Nat Commun; Sensory neurons regulate stimulus-dependent humoral immunity in mouse models of bacterial infection and asthma; Authors - Diane Aguilar

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

VIP (in red) worked nicely on IF in human paraffin brain sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

VIP Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:16233-1-AP)