VIPAS39 Rekombinanter Antikörper

VIPAS39 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241616A12

Kat-Nr. : 84320-2-RR

Synonyme

C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39

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  WB analysis using 84320-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 84320-2-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using VIPAS39 antibody (84320-2-RR, Clone: 241616A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84320-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, L02-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84320-2-RR bindet in WB, IF/ICC, ELISA VIPAS39 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VIPAS39 fusion protein Ag14762
• Vollständiger Name: chromosome 14 open reading frame 133
• Berechnetes Molekulargewicht: 493 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC015054
• Gene symbol: VIPAS39
• Gene ID (NCBI): 63894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).