VIRMA/KIAA1429 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VIRMA/KIAA1429 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30166-1-PBS

Synonyme

KIAA1429, VIRMA, MSTP054, virilizer

---

• 

  WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 30166-1-AP (same clone as 30166-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 30166-1-AP (same clone as 30166-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30166-1-AP (VIRMA/KIAA1429 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 30166-1-AP (same clone as 30166-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VIRMA/KIAA1429 antibody (30166-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30166-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

30166-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VIRMA/KIAA1429 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VIRMA/KIAA1429 fusion protein Ag32830
• Vollständiger Name: KIAA1429
• Beobachtetes Molekulargewicht: 200-210 kDa
• GenBank-Zugangsnummer: BC003701
• Gene symbol: KIAA1429
• Gene ID (NCBI): 25962
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VIRMA (KIAA1429) is a key component of m6A methyltransferase (writer) complex that include METTL3, METTL14, WTAP, VIRMA, HAKAI, ZC3H13, and RBM15. VIRMA mediates methylation in the 3'UTR and around the stop codon, thus affecting alternative polyadenylation. VIRMA has been reported to act as an oncogenic factor in breast cancer and liver cancer. KIAA1429 exhibits differential nuclear and cytoplasmic distribution in colorectal cancer samples, and high cytoplasmic expression of KIAA1429 is associated with poor response to chemotherapy. BRAF-mediated phosphorylation of KIAA1429 facilitates its aggregation in the cytoplasm. Furthermore, cytoplasmic KIAA1429 promotes WNT pathway activation by binding and stabilizing FZD7, thereby further enhancing cancer stemness and oxaliplatin resistance (PMID: 40611274). Moreover, this antibody can not recognize short isoforms.