VLDLR Rekombinanter Antikörper

VLDLR Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242385H3

Kat-Nr. : 85097-1-PBS

Synonyme

Very low-density lipoprotein receptor, VLDL receptor, VLDL-R

---

• 

  WB analysis using 85097-1-RR (same clone as 85097-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85097-1-RR (VLDLR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85097-1-PBS in a different storage buffer formulation.

• 

  FC experiment of THP-1 using 85097-1-RR (same clone as 85097-1-PBS)

  1x10^6 THP-1 cells were intracellularly stained with 0.25 ug VLDLR Recombinant Antibody (85097-1-RR, Clone:242385H3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85097-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

85097-1-PBS bindet in WB, FC (Intra), Indirect ELISA VLDLR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: very low density lipoprotein receptor
• Berechnetes Molekulargewicht: 96 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: NM_003383
• Gene symbol: VLDLR
• Gene ID (NCBI): 7436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family with a complex molecular weight profile due to post-translational modifications. The calculated molecular weight of the core protein is approximately 84-86 kDa, however, VLDLR undergoes extensive glycosylation during processing, resulting in higher apparent molecular weights observed in SDS-PAGE analyses. Multiple studies report that glycosylated VLDLR migrates as broad bands between 90-180 kDa under reducing conditions, with the most commonly observed forms at approximately 96 kDa (core glycosylated form), 115-135 kDa (mature fully glycosylated form), and 150-180 kDa (hyperglycosylated or dimeric forms).