VLGR1 Polyklonaler Antikörper

VLGR1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31628-1-PBS

Synonyme

GPR98, FEB4, G protein coupled receptor 98, KIAA0686, MASS1

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  WB analysis using 31628-1-AP (same clone as 31628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 31628-1-AP (same clone as 31628-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31628-1-AP (VLGR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

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  IP experiment of SH-SY5Y using 31628-1-AP (same clone as 31628-1-PBS)

  IP result of anti-VLGR1 (IP:31628-1-AP, 4ug; Detection:31628-1-AP 1:500) with SH-SY5Y cells lysate 1920 ug. This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 31628-1-AP (same clone as 31628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31628-1-AP (VLGR1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31628-1-AP (same clone as 31628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31628-1-AP (VLGR1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31628-1-AP (same clone as 31628-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31628-1-AP (VLGR1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 31628-1-AP (same clone as 31628-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using VLGR1 antibody (31628-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31628-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31628-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA VLGR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VLGR1 fusion protein Ag34635
• Vollständiger Name: G protein-coupled receptor 98
• Berechnetes Molekulargewicht: 693 kDa
• Beobachtetes Molekulargewicht: 217 kDa
• GenBank-Zugangsnummer: NM_032119
• Gene symbol: GPR98
• Gene ID (NCBI): 84059
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VLGR1 (very large G protein-coupled receptor-1), also known as ADGRV1, GPR98, and MASS, has a molecular weight of up to 700 kDa, making it the largest member of the 33 adhesive G protein-coupled receptors (ADGRs), a unique subfamily of the GPCR superfamily. The full-length VLGR1 encompasses ∼6300 amino acids and thus is difficult to analyze by electrophoresis. Vlgr1 is a core component of ankle-link complex in inner ear hair cells. Knock-out and mutation mouse models show that loss of Vlgr1 function leads to abnormal stereociliary development and hearing loss, indicating crucial roles of Vlgr1 in hearing transduction or auditory system development (PMID: 23180093). Western blot analysis detected VLGR1a isoform at an apparent molecular mass of 217 kDa .