VMAT2 Polyklonaler Antikörper

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• KD/KO Validated

VMAT2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20873-1-AP

Synonyme

Monoamine transporter, SLC18A2, SVAT, SVMT, VAT2, Vesicular amine transporter 2, VMAT2

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 20873-1-AP

  WB result of VMAT2 antibody (20873-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VMAT2 transfected Jurkat cells.

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  WB analysis of SGC-7901 using 20873-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 20873-1-AP (VMAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20873-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20873-1-AP (VMAT2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 20873-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20873-1-AP (VMAT2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 20873-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VMAT2 antibody (20873-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SGC-7901-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 4 publications below

Produktinformation

20873-1-AP bindet in WB, IHC, IF, ELISA VMAT2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VMAT2 fusion protein Ag14971
• Vollständiger Name: solute carrier family 18 (vesicular monoamine), member 2
• Berechnetes Molekulargewicht: 514 aa, 56 kDa
• Beobachtetes Molekulargewicht: 45-55 kDa
• GenBank-Zugangsnummer: BC108928
• Gene symbol: SLC18A2
• Gene ID (NCBI): 6571
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VMAT2 is a vesicular monoamine transporter that is responsible for the uptake and storage of monoamines (dopaine, norepinephrine, epinephrine, histamine and serotonin) in neurons, endocrine cells, and tumors deriving from these cells. Histamine-producing endocrine tumors (gastric carcinoids) expressed VMAT2 almost exclusively, thus VMAT2 is useful in classification of neuroendocrine tumors. Reduced expression of VMAT2 has often been observed in Parkinson's diseased (PD) brain, and detection of VMAT2 can be used to reflect the 4-(2-aminoethyl)benzene-1,2-diol neuron loss and predict the disease process. Several forms of VMAT2 can be observed upon the glycosylation: 45 kDa band corresponds to the native (deglycosylated) form, and the 55 and 75 kDa bands to glycosylated forms (PMID: 17582657).

Publikationen

Species	Application	Title
mouse	WB,IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human	IF	Nat Genet; Identification of TMEM230 mutations in familial Parkinson's disease.; Authors - Han-Xiang Deng
human	WB	Dis Model Mech; A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.; Authors - Zhao-Feng Li
human	IF	Front Neurosci; Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease; Authors - Fahad Mubariz
rat	WB	Parkinsons Dis; PMID: 30662707; Authors - Haruo Nishijima
human	IF	Mol Med; Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.; Authors - Sha Li