VNN1 Polyklonaler Antikörper

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VNN1 Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21745-1-PBS

Synonyme

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• 

  WB analysis of BxPC-3 using 21745-1-AP (same clone as 21745-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21745-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 21745-1-AP (same clone as 21745-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 21745-1-AP (same clone as 21745-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-13 using 21745-1-AP (same clone as 21745-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-13 using 21745-1-AP (same clone as 21745-1-PBS)

  PC-13 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21745-1-PBS in a different storage buffer formulation.

• 

  WB analysis of BxPC-3 using 21745-1-AP (same clone as 21745-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 21745-1-AP (VNN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21745-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21745-1-PBS bindet in WB, Indirect ELISA VNN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VNN1 fusion protein Ag16501
• Vollständiger Name: vanin 1
• Berechnetes Molekulargewicht: 513 aa, 57 kDa
• Beobachtetes Molekulargewicht: 55-72 kDa
• GenBank-Zugangsnummer: BC096268
• Gene symbol: VNN1
• Gene ID (NCBI): 8876
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VNN1(Vascular non-inflammatory molecule 1) is also named as Vanin-1, Pantetheinase, Tiff66. It is a GPI-anchored 70-kDa protein with a high degree of homology with GPI-80 (also known as VNN2), a molecule expressed by human phagocytes and involved in leukocyte adhesion and migration. Vanin-1 is predominantly expressed by resident tissue cells and acts at the level of epithelial cells and surrounding hematopoietic cells by paracrine 2-Mercaptoethylamine release(PMID:17163446). The full length VNN1 has a signal peptide, a propeptide and six glycosylation sites. According to some authors, the variations which varied between 55 and 72kDa may be due to different degrees of glycosylation of the protein and cleavage (JOSE A et al. 2001).