VPRBP Polyklonaler Antikörper

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• KD/KO Validated

VPRBP Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11612-1-PBS

Synonyme

DCAF1, DDB1- and CUL4-associated factor 1, EC:2.7.11.1, HIV 1 Vpr binding protein, HIV-1 Vpr-binding protein

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  WB analysis using 11612-1-AP (same clone as 11612-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11612-1-AP (same clone as 11612-1-PBS)

  WB result of VPRBP antibody (11612-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 11612-1-AP (same clone as 11612-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 11612-1-AP (same clone as 11612-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 11612-1-AP (same clone as 11612-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug. This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11612-1-AP (same clone as 11612-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11612-1-AP (VPRBP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (11612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 11612-1-AP (same clone as 11612-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug VPRBP Polyclonal antibody (11612-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11612-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11612-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA VPRBP und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPRBP fusion protein Ag2184
• Vollständiger Name: Vpr (HIV-1) binding protein
• Berechnetes Molekulargewicht: 1506 aa, 169 kDa
• Beobachtetes Molekulargewicht: 169 kDa
• GenBank-Zugangsnummer: BC022792
• Gene symbol: VPRBP
• Gene ID (NCBI): 9730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).