VPRBP Rekombinanter Antikörper

VPRBP Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241028B4

Kat-Nr. : 83901-5-PBS

Synonyme

241028B4, DCAF1, DDB1- and CUL4-associated factor 1, EC:2.7.11.1, HIV 1 Vpr binding protein

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  WB analysis using 83901-5-RR (same clone as 83901-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83901-5-RR (VPRBP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 83901-5-RR (same clone as 83901-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83901-5-RR (VPRBP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83901-5-RR (same clone as 83901-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (83901-5-RR, Clone: 241028B4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  FC experiment of U-2 OS using 83901-5-RR (same clone as 83901-5-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug VPRBP Recombinant antibody (83901-5-RR, Clone:241028B4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83901-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83901-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83901-5-RR against Human VPRBP were performed. The affinity constant is 44 pM.

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Geprüfte Anwendungen

Produktinformation

83901-5-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA VPRBP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VPRBP fusion protein Ag2184
• Vollständiger Name: Vpr (HIV-1) binding protein
• Berechnetes Molekulargewicht: 1506 aa, 169 kDa
• Beobachtetes Molekulargewicht: 169 kDa
• GenBank-Zugangsnummer: BC022792
• Gene symbol: VPRBP
• Gene ID (NCBI): 9730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507 amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).