VPS13A Polyklonaler Antikörper

VPS13A Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28618-1-PBS

Synonyme

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  WB analysis using 28618-1-AP (same clone as 28618-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 28618-1-AP (same clone as 28618-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 28618-1-AP (same clone as 28618-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28618-1-PBS bindet in WB, IHC, Indirect ELISA VPS13A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS13A fusion protein Ag29532
• Vollständiger Name: vacuolar protein sorting 13 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 360 kDa
• Beobachtetes Molekulargewicht: 360 kDa
• GenBank-Zugangsnummer: NM_033305
• Gene symbol: VPS13A
• Gene ID (NCBI): 23230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).