VPS13D Polyklonaler Antikörper

VPS13D Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 29387-1-AP

Synonyme

Intermembrane lipid transfer protein VPS13D, KIAA0453, Vacuolar protein sorting-associated protein 13D

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  WB analysis using 29387-1-AP

  HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 29387-1-AP (VPS13D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 29387-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29387-1-AP (VPS13D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 29387-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VPS13D antibody (29387-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

29387-1-AP bindet in WB, IHC, IF/ICC, ELISA VPS13D und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS13D fusion protein Ag29933
• Vollständiger Name: vacuolar protein sorting 13 homolog D (S. cerevisiae)
• Berechnetes Molekulargewicht: 492 kDa
• Beobachtetes Molekulargewicht: ~500 kDa
• GenBank-Zugangsnummer: NM_015378
• Gene symbol: VPS13D
• Gene ID (NCBI): 55187
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS13 (vacuolar protein sorting 13 ) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Lauren (Verified Customer) (11-12-2024)

Tested different dilutions of the antibody to determine which dilution resulted in the best stain index and this was the 1:200 dilution when using flow cytometry

• Applications: Flow Cytometry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: KG1a

VPS13D Antibody Flow Cytometry validation (1:200 dilution) in KG1a (Cat no:29387-1-AP)