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VPS13D Polyklonaler Antikörper

VPS13D Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 29387-1-PBS

Synonyme

Intermembrane lipid transfer protein VPS13D, KIAA0453, Vacuolar protein sorting-associated protein 13D

Filter:
  • Western Blot (WB) analysis of various lysates using VPS13D Polyclonal antibody (29387-1-AP)

    WB analysis using 29387-1-AP (same clone as 29387-1-PBS)

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 29387-1-AP (VPS13D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29387-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using VPS13D Polyclonal antibody (29387-1-AP)

    IHC staining of human ovary cancer using 29387-1-AP (same clone as 29387-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29387-1-AP (VPS13D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29387-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using VPS13D Polyclonal antibody (29387-1-AP)

    IF Staining of U2OS using 29387-1-AP (same clone as 29387-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using VPS13D antibody (29387-1-AP) at dilution of 1:200 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29387-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

29387-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VPS13D und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen VPS13D fusion protein Ag29933
Vollständiger Name vacuolar protein sorting 13 homolog D (S. cerevisiae)
Berechnetes Molekulargewicht 492 kDa
Beobachtetes Molekulargewicht~500 kDa
GenBank-ZugangsnummerNM_015378
Gene symbol VPS13D
Gene ID (NCBI) 55187
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS13 (vacuolar protein sorting 13 ) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).