VPS15 Polyklonaler Antikörper

VPS15 Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17894-1-PBS

Synonyme

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  WB analysis of HepG2 using 17894-1-AP (same clone as 17894-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 17894-1-AP (same clone as 17894-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17894-1-AP (VPS15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 17894-1-AP (same clone as 17894-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VPS15 antibody (17894-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 17894-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17894-1-PBS bindet in WB, IF/ICC, Indirect ELISA VPS15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS15 fusion protein Ag12221
• Vollständiger Name: phosphoinositide-3-kinase, regulatory subunit 4
• Berechnetes Molekulargewicht: 1358 aa, 153 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC110318
• Gene symbol: PIK3R4
• Gene ID (NCBI): 30849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PIK3R4(phosphoinositide 3-kinase regulatory subunit 4) is also named as PI3-kinase p150 subunit and belongs to the Ser/Thr protein kinase family. It is a regulatory subunit of the PI3K complex and may regulate membrane trafficking late in the endocytic pathway. This protein can be probably autophosphorylated(PMID:19369195).