VPS18 Polyklonaler Antikörper

VPS18 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10901-1-PBS

Synonyme

Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18

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  WB analysis of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10901-1-AP (VPS18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10901-1-AP (same clone as 10901-1-PBS)

  IP result of anti-VPS18 (IP:10901-1-AP, 4ug; Detection:10901-1-AP 1:300) with HeLa cells lysate 920ug. This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10901-1-AP (VPS18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 10901-1-AP (same clone as 10901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 10901-1-AP (same clone as 10901-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS18 antibody (10901-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10901-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA VPS18 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS18 fusion protein Ag1140
• Vollständiger Name: vacuolar protein sorting 18 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 110 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC001513
• Gene symbol: VPS18
• Gene ID (NCBI): 57617
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.