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VPS18 Monoklonaler Antikörper

VPS18 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E10G11

Kat-Nr. : 67590-1-PBS

Synonyme

hVPS18, KIAA1475, PEP3, VPS18

Filter:
  • Western Blot (WB) analysis of various lysates using VPS18 Monoclonal antibody (67590-1-Ig)

    WB analysis using 67590-1-Ig (same clone as 67590-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67590-1-Ig (VPS18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS18 Monoclonal antibody (67590-1-Ig)

    IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VPS18 Monoclonal antibody (67590-1-Ig)

    IHC staining of human liver cancer using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67590-1-Ig (VPS18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using VPS18 Monoclonal antibody (67590-1-Ig)

    IF Staining of A549 using 67590-1-Ig (same clone as 67590-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using VPS18 antibody (67590-1-Ig, Clone: 2E10G11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67590-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67590-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VPS18 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen VPS18 fusion protein Ag29954
Vollständiger Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 110 kDa
Beobachtetes Molekulargewicht 100-110 kDa
GenBank-ZugangsnummerBC001513
Gene symbol VPS18
Gene ID (NCBI) 57617
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.