VPS26A Polyklonaler Antikörper

VPS26A Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (2)

Kat-Nr. : 12804-1-AP

Synonyme

HB58, Hbeta58, hVPS26, PEP8A, Vesicle protein sorting 26A, VPS26, VPS26A

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Galerie der Validierungsdaten

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  WB analysis using 12804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12804-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12804-1-AP (VPS26A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12804-1-AP (VPS26A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12804-1-AP (VPS26A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12804-1-AP (VPS26A Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12804-1-AP (VPS26A Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12804-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12804-1-AP (VPS26A antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 12804-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12804-1-AP (VPS26A antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL555-phalloidine stains F-actin (red).

• 

  IF Staining of HeLa using 12804-1-AP

  Immunofluorescent analysis of HeLa cells using 12804-1-AP (VPS26A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12804-1-AP bindet in WB, IHC, IF, ELISA VPS26A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS26A fusion protein Ag3391
• Vollständiger Name: vacuolar protein sorting 26 homolog A (S. pombe)
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC022505
• Gene symbol: VPS26A
• Gene ID (NCBI): 9559
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1, and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

Publikationen

Species	Application	Title
human	IF	Cell Metab; Phospholipase PLA2G6, a Parkinsonism-Associated Gene, Affects Vps26 and Vps35, Retromer Function, and Ceramide Levels, Similar to α-Synuclein Gain.; Authors - Guang Lin
human	WB	Autophagy; RAB21 controls autophagy and cellular energy homeostasis by regulating retromer-mediated recycling of SLC2A1/GLUT1; Authors - Yifei Pei
human	WB,IHC	Int J Mol Sci; The Prognostic Value and the Oncogenic and Immunological Roles of Vacuolar Protein Sorting Associated Protein 26 A in Pancreatic Adenocarcinoma; Authors - Jihuan Hou ; KD Validated
human	WB	Ann Neurol; Retromer proteins reduced in Down syndrome and the Dp16 model: impact of APP dose and preclinical studies of a γ-secretase modulator; Authors - Xu-Qiao Chen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (10-10-2022)

It is Ok to detect endogenous VPS26A although there are some non-specific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

VPS26A Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:12804-1-AP)

FH

Stephen (Verified Customer) (09-07-2019)

Oc-2 cells fixed with 4% PFA Perm. by 0.3% tx100 for 5 min blocked with 1% BSA in 1XPBS for 2 hours vps26a antibody incubated 1:300 overnight at 4 degrees in 1%BSA in 1x PBS. Co-stained with DAPI (blue) and Ph647 (red) to visualize DNA and F-actin respectively.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: mouse OC2 cells

VPS26A Antibody Immunofluorescence, validation (1:300 dilution) in mouse OC2 cells (Cat no:12804-1-AP)