VPS26B Polyklonaler Antikörper

VPS26B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 15915-1-AP

Synonyme

Pep8b, Vesicle protein sorting 26B, VPS26B

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Galerie der Validierungsdaten

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  WB analysis using 15915-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15915-1-AP (VPS26B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15915-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15915-1-AP (VPS26B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 15915-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15915-1-AP (VPS26B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15915-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15915-1-AP (VPS26B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15915-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15915-1-AP (VPS26B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15915-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA VPS26B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS26B fusion protein Ag8708
• Vollständiger Name: vacuolar protein sorting 26 homolog B (S. pombe)
• Berechnetes Molekulargewicht: 336 aa, 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC009747
• Gene symbol: VPS26B
• Gene ID (NCBI): 112936
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

In mammals, there are two paralogues of yeast Vps26, VPS26A and VPS26B (PMID: 16190980). VPS26 is a component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. VPS26A and VPS26B subunits define distinct retromer complexes (PMID: 21920005). The retromer complex is important in recycling transmembrane receptors from endosomes to the trans-Golgi network (TGN).

Publikationen

Species	Application	Title
mouse	WB	Mol Psychiatry; Full recovery of the Alzheimer's disease phenotype by gain of function of vacuolar protein sorting 35.; Authors - Jian-Guo Li
mouse	WB	Nat Commun; Retromer stabilization results in neuroprotection in a model of Amyotrophic Lateral Sclerosis.; Authors - Luca Muzio
human	WB	Mol Psychiatry; VPS35 regulates tau phosphorylation and neuropathology in tauopathy.; Authors - Alana N Vagnozzi
rat	WB,CoIP,IF	Cell Death Differ; Sorting nexin 3 induces heart failure via promoting retromer-dependent nuclear trafficking of STAT3.; Authors - Jing Lu
human	WB, IP	Proc Natl Acad Sci U S A; A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.; Authors - Matthew Gallon
human	WB	Ann Neurol; Dysregulation of the Retromer Complex System in Down Syndrome.; Authors - Mary Elizabeth Curtis

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xin (Verified Customer) (10-10-2022)

It is reliable to detect VPS26B in mouse brain lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

VPS26B Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:15915-1-AP)