VPS28 Polyklonaler Antikörper

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• KD/KO Validated

VPS28 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 15478-1-AP

Synonyme

ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog

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  WB analysis of mouse brain using 15478-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15478-1-AP

  WB result of VPS28 antibody (15478-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS28 transfected HeLa cells.

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  WB analysis using 15478-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15478-1-AP

  IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IF Staining of HepG2 using 15478-1-AP

  Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Jurkat-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 3 publications below

Produktinformation

15478-1-AP bindet in WB, IF/ICC, IP, ELISA VPS28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS28 fusion protein Ag7784
• Vollständiger Name: vacuolar protein sorting 28 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC006485
• Gene symbol: VPS28
• Gene ID (NCBI): 51160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

Publikationen

Species	Application	Title
human	IF	PLoS Genet; AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.; Authors - Chiara Merigliano
human	WB,IF	iScience; VPS28 regulates brain vasculature by controlling neuronal VEGF trafficking through extracellular vesicle secretion.; Authors - Xiaohua Dong ; KD Validated
human	WB	Curr Protoc; Budget-Friendly Generation, Biochemical Analyses, and Lentiviral Transduction of Patient-Derived Colon Organoids; Authors - Emilie Rigaux
human	WB	J Extracell Biol; RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer; Authors - Yu-Chan Chang
human	WB,IF	Nat Commun; Rab41-mediated ESCRT machinery repairs membrane rupture by a bacterial toxin in xenophagy; Authors - Takashi Nozawa ; KD Validated