VPS28 Polyklonaler Antikörper

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• KD/KO Validated

VPS28 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15478-1-PBS

Synonyme

ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog

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  WB analysis of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15478-1-AP (same clone as 15478-1-PBS)

  WB result of VPS28 antibody (15478-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS28 transfected HeLa cells. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15478-1-AP (same clone as 15478-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15478-1-AP (VPS28 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 15478-1-AP (same clone as 15478-1-PBS)

  IP result of anti-VPS28 (IP:15478-1-AP, 4ug; Detection:15478-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 15478-1-AP (same clone as 15478-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 15478-1-AP (VPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15478-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15478-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA VPS28 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS28 fusion protein Ag7784
• Vollständiger Name: vacuolar protein sorting 28 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC006485
• Gene symbol: VPS28
• Gene ID (NCBI): 51160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.