VPS28 Rekombinanter Antikörper

VPS28 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

241886D3

Kat-Nr. : 84472-4-RR

Synonyme

Vacuolar protein sorting-associated protein 28 homolog, H-Vps28, ESCRT-I complex subunit VPS28, 241886D3

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  WB analysis using 84472-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 84472-4-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of SH-SY5Y using 84472-4-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug VPS28 Recombinant antibody (84472-4-RR, Clone:241886D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84472-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84472-4-RR against Human VPS28 were performed. The affinity constant is 1.72 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen, THP-1-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84472-4-RR bindet in WB, IF/ICC, FC (Intra), ELISA VPS28 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VPS28 fusion protein Ag7784
• Vollständiger Name: vacuolar protein sorting 28 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC006485
• Gene symbol: VPS28
• Gene ID (NCBI): 51160
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.