VPS33B Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS33B Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (1)

Kat-Nr. : 12195-1-AP

Synonyme

hVPS33B, VPS33B

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12195-1-AP

  WB result of VPS33B antibody (12195-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS33B transfected HEK-293 cells.

• 

  WB analysis of mouse testis using 12195-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12195-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12195-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human medulloblastoma using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12195-1-AP (VPS33B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human ovary using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 12195-1-AP

  Immunofluorescent analysis of MCF-7 cells, using VPS33B antibody 12195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, L02-Zellen, Maushodengewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Eierstockgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Medulloblastom-Gewebe, humanes Milzgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12195-1-AP bindet in WB, IP, IHC, IF, CoIP, ChIP, ELISA VPS33B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS33B fusion protein Ag2833
• Vollständiger Name: vacuolar protein sorting 33 homolog B (yeast)
• Berechnetes Molekulargewicht: 617 aa, 71 kDa
• Beobachtetes Molekulargewicht: 65-71 kDa
• GenBank-Zugangsnummer: BC016445
• Gene symbol: VPS33B
• Gene ID (NCBI): 26276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Publikationen

Species	Application	Title
human,mouse	WB,IF,IP	Nat Genet; Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.; Authors - Cullinane Andrew R AR ; KD Validated
mouse	WB	Nat Cell Biol; Circadian control of the secretory pathway maintains collagen homeostasis.; Authors - Joan Chang
human	WB,IF	Proc Natl Acad Sci U S A; Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.; Authors - Wong Dennis D
human	IF	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R
human	WB	Clin Kidney J; Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.; Authors - Amelia Holme
human	IHC, WB, CoIP, ChIP	Cancer Sci; VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.; Authors - Yingxia Ning

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RICHA (Verified Customer) (11-03-2020)

Excellent antibody for wester and IF.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

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