VPS37A Monoklonaler Antikörper

VPS37A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F9A4

Kat-Nr. : 66327-1-PBS

Synonyme

ESCRT I complex subunit VPS37A, HCRP1, hVps37A, PQBP2, VPS37A

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  WB analysis of SW 1990 using 66327-1-Ig (same clone as 66327-1-PBS)

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66327-1-Ig (VPS37A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66327-1-Ig (VPS37A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66327-1-Ig (same clone as 66327-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 66327-1-Ig (VPS37A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66327-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66327-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VPS37A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VPS37A fusion protein Ag19108
• Vollständiger Name: vacuolar protein sorting 37 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC022363
• Gene symbol: VPS37A
• Gene ID (NCBI): 137492
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.