VPS37C Polyklonaler Antikörper

VPS37C Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27128-1-PBS

Synonyme

ESCRT I complex subunit VPS37C, ESCRT-I complex subunit VPS37C, hVps37C, PML39, Vacuolar protein sorting-associated protein 37C

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  WB analysis using 27128-1-AP (same clone as 27128-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27128-1-AP (VPS37C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27128-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 27128-1-AP (same clone as 27128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27128-1-AP (VPS37C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27128-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 27128-1-AP (same clone as 27128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 27128-1-AP (VPS37C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27128-1-PBS in a different storage buffer formulation.

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  IF Staining of Raji using 27128-1-AP (same clone as 27128-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using VPS37C antibody (27128-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27128-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27128-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA VPS37C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS37C fusion protein Ag26024
• Vollständiger Name: vacuolar protein sorting 37 homolog C (S. cerevisiae)
• Beobachtetes Molekulargewicht: 39-45 kDa
• GenBank-Zugangsnummer: BC141827
• Gene symbol: VPS37C
• Gene ID (NCBI): 55048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS37C (Vacuolar protein sorting-associated protein 37C) is also named as hVps37C, PML39 and ESCRT-I complex subunit VPS37C. VPS37C is a core subunit of the Endosomal sorting complex required for transport-I (ESCRT-I) complex (PMID: 37307706). VPS37C interacts with EKLF and prevents K48-linked polyubiquitination of EKLF and proteasome-mediated EKLF degradation, thus enhancing EKLF protein stability and transcriptional activity (PMID: 37307706).