VPS39 Polyklonaler Antikörper

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• KD/KO Validated

VPS39 Polyklonal Antikörper für IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 16219-1-AP

Synonyme

hVam6p, KIAA0770, TLP, VAM6, Vam6/Vps39 like protein, VPS39

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Galerie der Validierungsdaten

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  IP experiment of mouse brain using 16219-1-AP

  IP Result of anti-VPS39 (IP:16219-1-AP, 4ug; Detection:16219-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human small intestine using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16219-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16219-1-AP (VPS39 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Dünndarmgewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Produktinformation

16219-1-AP bindet in WB, IP, IHC, IF, ELISA VPS39 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS39 fusion protein Ag9267
• Vollständiger Name: vacuolar protein sorting 39 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 886 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: BC015817
• Gene symbol: VPS39
• Gene ID (NCBI): 23339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS39, also named as KIAA0770, TLP, or VAM6, is a 886 amino acid protein, which contains one CNH domain and one CHCR repeat. VPS39 belongs to the VAM6/VPS39 family and localizes in the cytoplasm. VPS39 is widely expressed, with highest levels in heart, skeletal muscle, kidney, pancreas, brain, placenta and spleen. VPS39 may play a role in clustering and fusion of late endosomes and lysosomes. VPS39 modulates the transforming growth factor-beta response by coupling the transforming growth factor-beta receptor complex to the Smad pathway.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
	IP	Cell Host Microbe; Global mapping of Salmonella enterica-host protein-protein interactions during infection.; Authors - Philipp Walch
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	IF,WB	Dev Cell; ORF3a of SARS-CoV-2 promotes lysosomal exocytosis-mediated viral egress; Authors - Di Chen ; KD Validated
human	WB	Curr Biol; Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.; Authors - Anna Ulbricht
human,mouse	WB,IF	Mol Metab; SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells.; Authors - Joshua Jackson