VPS45 Polyklonaler Antikörper

VPS45 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Rezensionen (1)

Kat-Nr. : 12006-1-AP

Synonyme

Vacuolar protein sorting-associated protein 45, h-VPS45, hlVps45, H1VPS45, H1

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  IHC staining of mouse brain using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12006-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 12006-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12006-1-AP

  Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Herzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

12006-1-AP bindet in IHC, IF/ICC, ELISA VPS45 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS45 fusion protein Ag2658
• Vollständiger Name: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 570 aa, 65 kDa
• GenBank-Zugangsnummer: BC012932
• Gene symbol: VPS45
• Gene ID (NCBI): 11311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Publikationen

Species	Application	Title
		Gene; Rice protein suppresses ROS generation and stimulates antioxidant gene expression via Nrf2 activation in adult rats.; Authors - Hui Li
human	IHC	Mol Carcinog; VPS45 Contributes to the Progression of Hepatocellular Carcinoma by Triggering the Wnt/β-Catenin Signaling Pathway; Authors - Renhou Zhi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jerome (Verified Customer) (05-28-2020)

Very inconclusive, a lot of background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 100
• Cell Tissue Type: HeLa

VPS45 Antibody Immunofluorescence validation (100 dilution) in HeLa (Cat no:12006-1-AP)