VPS45 Polyklonaler Antikörper

VPS45 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12006-1-PBS

Synonyme

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  IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12006-1-AP (same clone as 12006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12006-1-AP (same clone as 12006-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12006-1-PBS bindet in IHC, IF/ICC, Indirect ELISA VPS45 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS45 fusion protein Ag2658
• Vollständiger Name: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 570 aa, 65 kDa
• GenBank-Zugangsnummer: BC012932
• Gene symbol: VPS45
• Gene ID (NCBI): 11311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)