VPS45 Polyklonaler Antikörper

VPS45 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12616-1-AP

Synonyme

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A

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Galerie der Validierungsdaten

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  WB analysis of mouse liver using 12616-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12616-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12616-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12616-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12616-1-AP

  IP result of anti-VPS45 (IP:12616-1-AP, 4ug; Detection:12616-1-AP 1:300) with mouse kidney tissue lysate 3000 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Nierengewebe, Maushirngewebe, Ratten-Pankreasgewebe
• Erfolgreiche IP: Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12616-1-AP bindet in WB, IP, ELISA VPS45 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS45 fusion protein Ag3289
• Vollständiger Name: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 570 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC028382
• Gene symbol: VPS45
• Gene ID (NCBI): 11311
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Publikationen

Species	Application	Title
human	WB	J Cell Sci; The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.; Authors - Laufman Orly O