VPS4A Polyklonaler Antikörper

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• KD/KO Validated

VPS4A Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14272-1-PBS

Synonyme

EC:3.6.4.6, hVPS4, Protein SKD2, SKD1, SKD2

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  WB analysis using 14272-1-AP (same clone as 14272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 14272-1-AP (same clone as 14272-1-PBS)

  WB result of VPS4A antibody (14272-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS4A transfected U-937 cells. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14272-1-AP (same clone as 14272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 14272-1-AP (same clone as 14272-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 14272-1-AP (same clone as 14272-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 14272-1-AP (same clone as 14272-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14272-1-AP (VPS4A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14272-1-AP (same clone as 14272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 14272-1-AP (same clone as 14272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14272-1-AP (VPS4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14272-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14272-1-PBS bindet in WB, IHC, Indirect ELISA VPS4A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS4A fusion protein Ag5592
• Vollständiger Name: vacuolar protein sorting 4 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC047932
• Gene symbol: VPS4A
• Gene ID (NCBI): 27183
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vacuolar Protein Sorting 4A (VPS4A) is one of two human homologs of the yeast protein Vps4 and member of the AAA protein family (ATPases associated with diverse cellular activities).