VPS4A/B Polyklonaler Antikörper

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• KD/KO Validated

VPS4A/B Polyklonal Antikörper für WB, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17673-1-PBS

Synonyme

VPS4B, Vacuolar protein sorting-associated protein 4B, SKD1, Protein SKD1, MIG1

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  WB analysis using 17673-1-AP (same clone as 17673-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4A/B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skin using 17673-1-AP (same clone as 17673-1-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 17673-1-AP (VPS4B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 17673-1-AP (same clone as 17673-1-PBS)

  IP result of anti-VPS4A/B (IP:17673-1-AP, 4ug; Detection:17673-1-AP 1:3000) with HeLa cells lysate 1360 ug. This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 17673-1-AP (same clone as 17673-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug VPS4A/B Polyclonal antibody (17673-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 17673-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17673-1-PBS bindet in WB, FC (Intra), IP, Indirect ELISA VPS4A/B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS4A/B fusion protein Ag11610
• Vollständiger Name: vacuolar protein sorting 4 homolog B (S. cerevisiae)
• Berechnetes Molekulargewicht: 444 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC039574
• Gene symbol: VPS4B
• Gene ID (NCBI): 9525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. Human VPS4 has two orthologous proteins named VPS4A and VPS4B. This antibody can recognize both VPS4A and VPS4B due to their high homology.