VPS54 Polyklonaler Antikörper

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VPS54 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13327-1-PBS

Synonyme

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

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  WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 13327-1-AP (same clone as 13327-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 13327-1-AP (VPS54 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 13327-1-AP (same clone as 13327-1-PBS)

  IP result of anti-VPS54 (IP:13327-1-AP, 4ug; Detection:13327-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13327-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13327-1-PBS bindet in WB, IP, Indirect ELISA VPS54 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS54 fusion protein Ag4123
• Vollständiger Name: vacuolar protein sorting 54 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 977 aa, 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa
• GenBank-Zugangsnummer: BC030275
• Gene symbol: VPS54
• Gene ID (NCBI): 51542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.