VPS54 Monoklonaler Antikörper

VPS54 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D12D1

Kat-Nr. : 67404-1-PBS

Synonyme

Vacuolar protein sorting-associated protein 54, Tumor antigen SLP-8p, Tumor antigen HOM-HCC-8, Hepatocellular carcinoma protein 8, HCC8

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  WB analysis of SH-SY5Y using 67404-1-Ig (same clone as 67404-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67404-1-Ig (same clone as 67404-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67404-1-Ig (VPS54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67404-1-Ig (same clone as 67404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67404-1-Ig (VPS54 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67404-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51221-2

  Cytometric bead array standard curve of MP51221-2, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-2-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-25 ng/mL.

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  Cytometric bead array standard curve of MP51221-3

  Cytometric bead array standard curve of MP51221-3, VPS54 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67404-3-PBS. Detection antibody: 67404-1-PBS. Standard:Ag4123. Range: 0.195-12.5 ng/mL.

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Geprüfte Anwendungen

Produktinformation

67404-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA VPS54 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: VPS54 fusion protein Ag4123
• Vollständiger Name: vacuolar protein sorting 54 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 977 aa, 111 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC030275
• Gene symbol: VPS54
• Gene ID (NCBI): 51542
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS54 is a part of the Golgi-associated retrograde protein (GARP) complex protein required for tethering and fusion of endosome-derived transport vesicles to the trans-Golgi network. It may particpate in retrograde transport from early and late endosomes to the late Golgi. The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD.