VRK2 Polyklonaler Antikörper

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• KD/KO Validated

VRK2 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 12946-1-AP

Synonyme

Vaccinia-related kinase 2, vaccinia related kinase 2, Serine/threonine-protein kinase VRK2, EC:2.7.11.1

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  WB analysis using 12946-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12946-1-AP

  WB result of VRK2 antibody (12946-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VRK2 transfected HepG2 cells.

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  WB analysis of human liver using 12946-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12946-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12946-1-AP (VRK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 12946-1-AP

  IP result of anti-VRK2 (IP:12946-1-AP, 4ug; Detection:12946-1-AP 1:500) with K-562 cells lysate 3600ug.

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  IHC staining of human stomach using 12946-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12946-1-AP (VRK2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, BxPC-3-Zellen, humanes Lebergewebe, K-562-Zellen, Maus-Skelettmuskelgewebe, U2OS-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Produktinformation

12946-1-AP bindet in WB, IHC, IP, ELISA VRK2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VRK2 fusion protein Ag4019
• Vollständiger Name: vaccinia related kinase 2
• Berechnetes Molekulargewicht: 508 aa, 58 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC027854
• Gene symbol: VRK2
• Gene ID (NCBI): 7444
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vaccinia-related kinase 2 (VRK2) is a serine/threonine kinase that plays a significant role in various cellular processes, including cell survival, proliferation, and DNA damage response. The VRK2 gene is known to produce two main splice variants: VRK2A and VRK2B, with VRK2A being the predominant form in humans. VRK2 is involved in several key biological functions. It is known to enhance cell survival by acting as an anti-apoptotic factor, which is particularly crucial in cancer biology. The overexpression of VRK2 has been linked to increased drug sensitivity in cancer cells, suggesting that this kinase may play a role in therapeutic responses. Additionally, high levels of VRK2 protein have been associated with improved survival rates in specific subgroups of astrocytomas, a type of brain tumor. (PMID: 29872222; 37943248; 24079673)

Publikationen

Species	Application	Title
human	WB	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu
human	WB	Int J Biol Sci; VRK2 activates TNFα/NF-κB signaling by phosphorylating IKKβ in pancreatic cancer.; Authors - Jionghuang Chen
mouse	WB	BMC Med; Reduced Vrk2 expression is associated with higher risk of depression in humans and mediates depressive-like behaviors in mice; Authors - Mei-Yu Yin ; KO Validated
human	WB,IHC	Cell Rep; Elevated FBXW10 drives hepatocellular carcinoma tumorigenesis via AR-VRK2 phosphorylation-dependent GAPDH ubiquitination in male transgenic mice; Authors - Xiao-Tong Lin ; KD Validated