VILIP1 Polyklonaler Antikörper

VILIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13919-1-AP

Synonyme

VILIP 1, VILIP-1, VSNL1, Hippocalcin like protein 3, Hippocalcin-like protein 3

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  WB analysis using 13919-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13919-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse tongue using 13919-1-AP

  mouse tongue tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13919-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 13919-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 13919-1-AP (VILIP-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13919-1-AP

  IP result of anti-VILIP-1 (IP:13919-1-AP, 3ug; Detection:13919-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of Human bowens disease using 13919-1-AP

  Immunohistochemical analysis of paraffin-embedded human brown disease slide using 13919-1-AP (VILIP-1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 13919-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VILIP-1 antibody (13919-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, humanes Hirngewebe, Maus-Cerebellum-Gewebe, Mauszungengewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Human bowens disease; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

13919-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA VILIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VILIP1 fusion protein Ag4923
• Vollständiger Name: visinin-like 1
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-24 kDa
• GenBank-Zugangsnummer: BC022012
• Gene symbol: VSNL1
• Gene ID (NCBI): 7447
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	WB,IF	Free Radic Biol Med; Dimerization of visinin-like-protein 1 is regulated by oxidative stress and calcium and is a pathological hallmark of amyotrophic lateral sclerosis.; Authors - Martina P Liebl
mouse	WB	Front Cell Neurosci; Lack of Helios During Neural Development Induces Adult Schizophrenia-Like Behaviors Associated With Aberrant Levels of the TRIF-Recruiter Protein WDFY1.; Authors - Anna Sancho-Balsells
mouse	WB	Sci Rep; Proteomic profile of human sinoatrial and atrioventricular nodes in comparison to working myocardium; Authors - Agata Krawczyk-Ożóg