VSX2 Polyklonaler Antikörper

VSX2 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 25825-1-AP

Synonyme

Ceh-10 homeodomain-containing homolog, CHX10, Homeobox protein CHX10, HOX10, MCOP2

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  WB analysis of mouse eye using 25825-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 25825-1-AP (VSX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human retinoblastoma using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human retinoblastoma using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human retinoblastoma tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 25825-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25825-1-AP (VSX2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 25825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 25825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using VSX2 antibody (25825-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Augengewebe
• Erfolgreiche Detektion in IHC: humanes Retinoblastom-Gewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

25825-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA VSX2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VSX2 fusion protein Ag23035
• Vollständiger Name: visual system homeobox 2
• Berechnetes Molekulargewicht: 361 aa, 39 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC128153
• Gene symbol: VSX2
• Gene ID (NCBI): 338917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cell Stem Cell; Human brain organoids assemble functionally integrated bilateral optic vesicles.; Authors - Elke Gabriel
human	IF	Nat Protoc; Generation of iPSC-derived human forebrain organoids assembling bilateral eye primordia; Authors - Elke Gabriel
mouse	IF	Pharmacol Res; NAALAD2 mutations disrupt the fate of photoreceptor cells and retinal pigment epithelial cells during early retinal development; Authors - Yuanjie Qian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence