VTA1 Polyklonaler Antikörper

VTA1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15786-1-AP

Synonyme

C6orf55, Dopamine-responsive gene 1 protein, DRG 1, DRG1, DRG-1

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  WB analysis using 15786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15786-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15786-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15786-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15786-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human urine using 15786-1-AP

  human urine sample were subjected to SDS PAGE followed by western blot with 15786-1-AP (VTA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15786-1-AP

  IP result of anti-VTA1 (IP:15786-1-AP, 4ug; Detection:15786-1-AP 1:10000) with HEK-293 cells lysate 1440 ug.

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  IHC staining of mouse stomach using 15786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 15786-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 15786-1-AP (VTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15786-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VTA1 antibody (15786-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A549-Zellen, fetales humanes Hirngewebe, HepG2-Zellen, humane Urinprobe, K-562-Zellen, Maushirngewebe, Rattenhirngewebe, SMMC-7721-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15786-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA VTA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VTA1 fusion protein Ag8485
• Vollständiger Name: Vps20-associated 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 307 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC006989
• Gene symbol: VTA1
• Gene ID (NCBI): 51534
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Proteomic analyses uncover a new function and mode of action for mDia2.; Authors - Tadamoto Isogai