VTI1B Polyklonaler Antikörper

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• KD/KO Validated

VTI1B Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14495-1-PBS

Synonyme

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  WB analysis using 14495-1-AP (same clone as 14495-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 14495-1-AP (same clone as 14495-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 14495-1-AP (same clone as 14495-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 14495-1-AP (same clone as 14495-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14495-1-AP (same clone as 14495-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14495-1-AP (VTI1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 14495-1-AP (same clone as 14495-1-PBS)

  IP result of anti-VTI1B (IP:14495-1-AP, 3ug; Detection:14495-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14495-1-AP (VTI1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human malignant melanoma using 14495-1-AP (same clone as 14495-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma using 14495-1-AP (VTI1B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14495-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14495-1-PBS bindet in WB, IHC, IP, Indirect ELISA VTI1B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VTI1B fusion protein Ag5906
• Vollständiger Name: vesicle transport through interaction with t-SNAREs homolog 1B (yeast)
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC003142
• Gene symbol: VTI1B
• Gene ID (NCBI): 10490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fusion between membranes is mediated by specific SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) complexes. Two human SNARE proteins, VTI1A and VTI1B, are homologous to the yeast Q-SNARE Vtilp which is part of several SNARE complexes in different transport steps (PMID: 12067063). Both proteins had a distinct but overlapping localization. VTI1A is localized predominantly in the TGN, VTI1B in late endosomes (PMID:12067063; 21262811). VTI1B forms a SNARE complex with STX7, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. It is a component of the SNARE complex composed of STX7, STX8, VAMP7 and VIT1B that is required for heterotypic fusion of late endosomes with lysosomes. It has also been reported that VIT1B interacts with EpsinR, a protein involved in exocytic trafficking (PMID: 15371541).