Vitronectin/S-protein Polyklonaler Antikörper

Vitronectin/S-protein Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (1)

Kat-Nr. : 15833-1-AP

Synonyme

Serum-spreading factor, Somatomedin-B, S-protein, V75, Vitronectin

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• 

  WB analysis of human plasma using 15833-1-AP

  human serum was subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15833-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15833-1-AP

  rat plasma was subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15833-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15833-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15833-1-AP (Vitronectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 15833-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15833-1-AP (Vitronectin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 15833-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15833-1-AP (Vitronectin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: rat plasma, humanes Hodengewebe, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Leberzirrhosegewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Produktinformation

15833-1-AP bindet in WB, IHC, IF/ICC, ELISA Vitronectin/S-protein und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Plasmodium falciparum
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Vitronectin/S-protein fusion protein Ag8443
• Vollständiger Name: vitronectin
• Berechnetes Molekulargewicht: 478 aa, 54 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa, 65 kDa,
• GenBank-Zugangsnummer: BC005046
• Gene symbol: VTN
• Gene ID (NCBI): 7448
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vitronectin is a 75-kDa glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).

Publikationen

Species	Application	Title
		Cell Stem Cell; Region-specific cellular and molecular basis of liver regeneration after acute pericentral injury; Authors - Shuyong Wang
rat	WB,IF	Brain Pathol; Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.; Authors - Hongjin Wang
human	WB,IF	Sci Rep; Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.; Authors - Takahiro Tougan
human	WB,IF	J Cell Mol Med; Induction of migration of periodontal ligament cells by selective regulation of integrin subunits.; Authors - Mari Kawamura
human		Langmuir; Characterization of Initial Cell Adhesion on Charged Polymer Substrates in Serum-Containing and Serum-Free Media.; Authors - Takashi Hoshiba
human	WB	Proteomics; SILAC-based Quantitative Proteomic Analysis of Secretome between Activated and Reverted Hepatic Stellate Cells.; Authors - Hongyu Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jingwen (Verified Customer) (01-28-2020)

It worked well when I performed the western blot in cancer samples.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Cancer tissue