VWF, VWFpp Polyklonaler Antikörper

VWF, VWFpp Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

95

Rezensionen (1)

Kat-Nr. : 11778-1-AP

Synonyme

F8VWF, von Willebrand antigen II, von Willebrand factor, VWD, VWF, VWF, VWFpp

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Galerie der Validierungsdaten

• 

  WB analysis using 11778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11778-1-AP (VWF, VWFpp antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 11778-1-AP

  IP Result of anti-VWF, VWFpp (IP:11778-1-AP, 4ug; Detection:11778-1-AP 1:300) with mouse spleen tissue lysate 5200ug.

• 

  IP experiment of mouse spleen using 11778-1-AP

  IP Result of anti-VWF, VWFpp (IP:11778-1-AP, 4ug; Detection:11778-1-AP 1:300) with mouse spleen tissue lysate 5200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below

Produktinformation

11778-1-AP bindet in WB, IP, ELISA VWF, VWFpp und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VWF, VWFpp fusion protein Ag2386
• Vollständiger Name: von Willebrand factor
• Berechnetes Molekulargewicht: 309 kDa
• Beobachtetes Molekulargewicht: 309-320 kDa, 75-100 kDa
• GenBank-Zugangsnummer: .
• Gene symbol: VWF
• Gene ID (NCBI): 7450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Von Willebrand factor (vWF) is a large multimeric glycoprotein that is crucial to the hemostasis process. It supports platelet adhesion and carries factor VIII (FVIII). Deficiency of vWF results in von Willebrand disease (vWD), a common inherited bleeding disorder. The pre-pro-vWF is comprised of 2,813 amino acids (aa) which encompass a 22-aa signal peptide, a 741-aa large propeptide (vWF propeptide, vWFpp, also called von Willebrand antigen 2) and 2,050 aa making up the mature vWF. The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. This antibody raised against the N-terminal region of pre-pro-vWF recognizes von Willebrand antigen 2 (75-100 kDa) and pro-vWF (309-320 kDa). (PMID: 9759493; 12067899; 22452980; 8874191)

Publikationen

Species	Application	Title
mouse		J Thromb Haemost; Knock-in mice bearing constitutively active αIIb(R990W) mutation develop macrothrombocytopenia with severe platelet dysfunction.; Authors - Keigo Akuta
mouse		Circulation; Hyperglycemia inhibits cardiac stem cell-mediated cardiac repair and angiogenic capacity.; Authors - André S D Molgat
pig		Circulation; Intramyocardial peptide nanofiber injection improves postinfarction ventricular remodeling and efficacy of bone marrow cell therapy in pigs.; Authors - Lin Yi-Dong YD
mouse		Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
rat		Adv Healthc Mater; A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction.; Authors - Zhi Zheng
rat		ACS Appl Mater Interfaces; Aligned Graphene Mesh-Supported Double Network Natural Hydrogel Conduit Loaded with Netrin-1 for Peripheral Nerve Regeneration.; Authors - Qun Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kim-Anh (Verified Customer) (09-03-2019)

Good primary antibody, I used for IF test on HUVECs

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: huvecs

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