Serpin A12/Vaspin Monoklonaler Antikörper

Serpin A12/Vaspin Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A1B6

Kat-Nr. : 67573-1-Ig

Synonyme

SERPINA12, SERPINA12/Vaspin, Vaspin, 2A1B6, OL 64

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  WB analysis using 67573-1-Ig

  Human adipose tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67573-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67573-1-Ig (Vaspin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 67573-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vaspin antibody (67573-1-Ig, Clone: 2A1B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Fettgewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67573-1-Ig bindet in WB, IF/ICC, ELISA Serpin A12/Vaspin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Serpin A12/Vaspin fusion protein Ag30136
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
• Berechnetes Molekulargewicht: 414 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC040857
• Gene symbol: Vaspin
• Gene ID (NCBI): 145264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA12 is also named as Serpin A12 and Visceral adipose tissue-derived serine protease inhibitor (Vaspin). Vaspin is an adipokine belonging to the serpin family, thus also named SERPINA12, with targets in kallikrein 7 and 14 that participates in skin desquamation (PMID: 34359881). Vaspin is a compensatory molecule in obesity and insulin resistance (PMID: 18321232). Vaspin is a newly described adipokine with an insulin sensitizing effect and inhibitory impact on food intake (PMID: 16030142, PMID: 21465327).