VEGFA Polyklonaler Antikörper

VEGFA Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Kat-Nr. : 26157-1-AP

Synonyme

L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A, Vascular endothelial growth factor A, long form

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  WB analysis of mouse brain using 26157-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 26157-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 26157-1-AP (Vegfa antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse lung using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26157-1-AP (Vegfa antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse kidney using 26157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26157-1-AP (VEGFA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

26157-1-AP bindet in WB, IHC, IF, ELISA VEGFA und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Ag24084
• Vollständiger Name: vascular endothelial growth factor A
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_001287056
• Gene symbol: Vegfa
• Gene ID (NCBI): 22339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.

Publikationen

Species	Application	Title
mouse	WB	Carbohydr Polym; Carboxymethyl chitosan and sodium alginate oxide pH-sensitive dual-release hydrogel for diabetes wound healing: The combination of astilbin liposomes and diclofenac sodium; Authors - Xiaoyu Wu
rat	WB	Respir Res; The role of RNA m6A methylation in the regulation of postnatal hypoxia-induced pulmonary hypertension.; Authors - Shanshan Xu
human	WB	Aging (Albany NY); Transplanting Rac1-silenced bone marrow mesenchymal stem cells promote neurological function recovery in TBI mice.; Authors - Dongdong Huang
mouse	WB	Cell Biol Int; Involvement of HIF1 stabilization and VEGF signaling modulated by Grx-1 in murine model of bronchopulmonary dysplasia; Authors - Wenli Han
human	WB	Cancer Biol Ther; RSK4 promotes the metastasis of clear cell renal cell carcinoma by activating RUNX1-mediated angiogenesis; Authors - Jing Ma
mouse	WB	Biochem Biophys Res Commun; Ablation of glutaredoxin 1 promotes pulmonary angiogenesis and alveolar formation in hyperoxia-injured lungs by modifying HIF-1α stability and inhibiting the NF-κB pathway.; Authors - Xuwei Liu