VEGFA Rekombinanter Antikörper

VEGFA Rekombinant Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2I18

Kat-Nr. : 82864-7-PBS

Synonyme

2I18, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

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  IHC staining of mouse lung using 82864-7-RR (same clone as 82864-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82864-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 82864-7-RR (same clone as 82864-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82864-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82864-7-RR (same clone as 82864-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82864-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 82864-7-RR (same clone as 82864-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82864-7-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 82864-7-RR (same clone as 82864-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Vegfa antibody (82864-7-RR, Clone: 2I18 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82864-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82864-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82864-7-RR against Mouse Vegfa were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82864-7-PBS bindet in IHC, IF/ICC, Indirect ELISA VEGFA und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Eg32035
• Vollständiger Name: vascular endothelial growth factor A
• GenBank-Zugangsnummer: NM_001287056
• Gene symbol: Vegfa
• Gene ID (NCBI): 22339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.