VEGFA Rekombinanter Antikörper

VEGFA Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2I18

Kat-Nr. : 82864-7-RR

Synonyme

2I18, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

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  IHC staining of mouse lung using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 82864-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82864-7-RR (VEGFA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 82864-7-RR

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Vegfa antibody (82864-7-RR, Clone: 2I18 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 82864-7-RR

  Biolayer interferometry (BLl) kinetic assays of 82864-7-RR against Mouse Vegfa were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mauslungengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82864-7-RR bindet in IHC, IF/ICC, ELISA VEGFA und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFA fusion protein Eg32035
• Vollständiger Name: vascular endothelial growth factor A
• GenBank-Zugangsnummer: NM_001287056
• Gene symbol: Vegfa
• Gene ID (NCBI): 22339
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vegfa is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis.It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. It may play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis.