Vimentin Rekombinanter Antikörper

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Vimentin Rekombinant Antikörper für WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

6K21

Kat-Nr. : 80232-1-PBS

Synonyme

VIM, 6K21

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  WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 80232-1-RR (same clone as 80232-1-PBS)

  WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IHC staining of human melanoma tissue using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human melanoma slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IHC staining of human melanoma tissue using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human melanoma tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80232-1-RR (same clone as 80232-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80232-1-RR (same clone as 80232-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80232-1-PBS bindet in WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA Vimentin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Vimentin fusion protein Ag0489
• Vollständiger Name: vimentin
• Berechnetes Molekulargewicht: 466 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000163
• Gene symbol: VIM
• Gene ID (NCBI): 7431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).