Vimentin Rekombinanter Antikörper

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• KD/KO Validated

Vimentin Rekombinant Antikörper für WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

6K21

Kat-Nr. : 80232-1-RR

Synonyme

VIM, 6K21

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  WB analysis using 80232-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 80232-1-RR

  WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells.

• 

  WB analysis using 80232-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human melanoma tissue using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human melanoma slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​

• 

  IHC staining of human melanoma tissue using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human melanoma tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​

• 

  IHC staining of human breast cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 80232-1-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse breast cancer using 80232-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HeLa using 80232-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, 4T1-Zellen, A549-Zellen, HeLa-Zellen, NIH/3T3-Zellen, ROS1728-Zellen, U-251-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC-Autostainer: human melanoma tissue
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Kolongewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-Fro: mouse breast cancer
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:20000-1:100000
• Immunhistochemie (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:250-1:1000
• Immunhistochemie (IHC): IHC : 1:1250-1:5000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 1 publications below

Produktinformation

80232-1-RR bindet in WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, ELISA Vimentin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Vimentin fusion protein Ag0489
• Vollständiger Name: vimentin
• Berechnetes Molekulargewicht: 466 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000163
• Gene symbol: VIM
• Gene ID (NCBI): 7431
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Publikationen

Species	Application	Title
human	WB	BMC Cancer; Comprehensive analysis of scRNA-seq and bulk RNA-seq data via machine learning and bioinformatics reveals the role of lysine metabolism-related genes in gastric carcinogenesis; Authors - Yongfu Shao
human	WB	J Exp Clin Cancer Res; A novel polypeptide encoded by circSPIRE1 promotes prostate cancer proliferation and migration by restraining the ubiquitin-dependent degradation of LRP5; Authors - Jintao Hu
human	WB	Front Immunol; The miR-941/FOXN4/TGF-β feedback loop induces N2 polarization of neutrophils and enhances tumor progression of lung adenocarcinoma; Authors - Xiaojing Zhang
human	WB,IF	J Neurooncol; The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells; Authors - Hui Xu
human	WB	Mol Cancer Res; Ubiquitin ligase TRIM22 inhibits ovarian cancer malignancy via TCF4 degradation; Authors - Tao Tao