Vimentin Monoklonaler Antikörper

Vimentin Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF, FC (Intra)

Konjugation

CoraLite®488 Fluorescent Dye

Publikationen

1

CloneNo.

3H9D1

Kat-Nr. : CL488-60330

Synonyme

VIM, vimentin

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Galerie der Validierungsdaten

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  IF Staining of HepG2 using CL488-60330

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®488 Vimentin antibody (CL488-60330, Clone: 3H9D1 ) at dilution of 1:400.

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  IF Staining of human tonsillitis using CL488-60330

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60330 (Vimentin antibody) at dilution of 1:100..

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  IF Staining of human tonsillitis using CL488-60330

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60330 (Vimentin antibody) at dilution of 1:100.

• 

  IF Staining of HepG2 using CL488-60330

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®488 Vimentin antibody (CL488-60330, Clone: 3H9D1 ) at dilution of 1:400.

• 

  FC experiment of Jurkat using CL488-60330

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Vimentin (CL488-60330, Clone:3H9D1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HepG2-Zellen, humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

CL488-60330 bindet in IHC, IF, FC (Intra) Vimentin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vimentin fusion protein Ag0489
• Vollständiger Name: vimentin
• Berechnetes Molekulargewicht: 466 aa, 54 kDa
• GenBank-Zugangsnummer: BC000163
• Gene symbol: VIM
• Gene ID (NCBI): 7431
• Konjugation: CoraLite®488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 491 nm / 516 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publikationen

Species	Application	Title
mouse	IHC, IF	J Mol Cell Biol; Cholesterol metabolic enzyme Ggpps regulates epicardium development and ventricular wall architecture integrity in mice.; Authors - Feng Zheng

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C