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Vimentin Rekombinanter Antikörper

Vimentin Rekombinant Antikörper für IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

6K21

Kat-Nr. : CL647-80232

Synonyme

FLJ36605, VIM, vimentin



Geprüfte Anwendungen

Erfolgreiche Detektion in IFHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-80232 bindet in IF Vimentin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Vimentin fusion protein Ag0489
Vollständiger Name vimentin
Berechnetes Molekulargewicht 466 aa, 54 kDa
Beobachtetes Molekulargewicht50-55 kDa
GenBank-ZugangsnummerBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 647 Vimentin antibody CL647-80232Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen