Vinculin Monoklonaler Antikörper
Vinculin Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (3)
Anwendung
WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2B5A7
Kat-Nr. : 66305-1-Ig
Synonyme
"Vinculin Antibodies" Comparison
View side-by-side comparison of Vinculin antibodies from other vendors to find the one that best suits your research needs.
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HepG2-Zellen, C6-Zellen, HEK-293-Zellen, HeLa-Zellen, HuH-7-Zellen, L02-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, Hausschwein-Herzgewebe, U-251-Zellen, U-87 MG-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, Mausherzgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 162 publications below |
IHC | See 2 publications below |
IF | See 34 publications below |
CoIP | See 1 publications below |
Produktinformation
66305-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA Vinculin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Kaninchen, Maus, Ratte, Zebrafisch |
Wirt / Isotyp | Maus / IgG1 |
Klonalität | Monoklonal |
Typ | Antikörper |
Immunogen | Vinculin fusion protein Ag24946 |
Vollständiger Name | vinculin |
Berechnetes Molekulargewicht | 1133 aa, 124 kDa |
Beobachtetes Molekulargewicht | 117 kDa |
GenBank-Zugangsnummer | BC039174 |
Gene symbol | Vinculin |
Gene ID (NCBI) | 7414 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Protein-G-Reinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for Vinculin antibody 66305-1-Ig | Protokoll herunterladen |
IHC protocol for Vinculin antibody 66305-1-Ig | Protokoll herunterladenl |
IF protocol for Vinculin antibody 66305-1-Ig | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Crit Care Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein. | ||
Adv Sci (Weinh) RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation | ||
Cell Rep Med Development of an orally bioavailable CDK12/13 degrader and induction of synthetic lethality with AKT pathway inhibition | ||
Dev Cell HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Monifa (Verified Customer) (08-31-2025) | I used vinculin as an alternative loading control to beta actin. After adjusting my transfer buffer to help vinculin transfer to my membrane, I can visualize the protein very well (with chemiluminescence).
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FH Aditya (Verified Customer) (01-31-2025) | very clean bands, much better than the abcam antibody
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FH Morgane (Verified Customer) (01-09-2025) | Very good loading control with larger molecular size
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FH Daniel (Verified Customer) (10-24-2024) | The antibody works really well and it gives a very clean Western blot.
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FH Lisa (Verified Customer) (04-29-2024) | Works super well!
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FH Parijat (Verified Customer) (08-21-2023) | Works well as loading control
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FH Udesh (Verified Customer) (08-16-2023) | Worked well in WB at 1:3000 and IF at 1:100
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FH Mohamad (Verified Customer) (07-03-2023) | Very good antibody
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FH Priya (Verified Customer) (01-17-2023) | I have used for human cardiomyocytes, mouse skin and liver tissues
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FH Macarena (Verified Customer) (10-07-2022) | excellent results.
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FH Jonas (Verified Customer) (07-29-2022) | This is my go to loading control stain to validate evenly loaded lanes Binding could be stronger but by using 1:500 dilution, a reliable staining can be achieved
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FH Charlotte (Verified Customer) (07-26-2022) | Very good antibody. Very specific, super fast to reveal. Here we see Gli1 (160 kDa) because it is a mouse antibody too.
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FH AKIMASA (Verified Customer) (04-28-2021) | I could get the good quality band!
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FH Chun (Verified Customer) (09-07-2020) | This is a fairly good antibody for immunoblotting.
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FH Huai-Chin (Verified Customer) (06-08-2019) | Serving as a loading control, this antibody is not that good compare to other. Still work to some extent.
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