Vinculin Monoklonaler Antikörper

Vinculin Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2B5A7

Kat-Nr. : 66305-1-PBS

Synonyme

VCL, MVCL, MV, Metavinculin, CMD1W

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  WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66305-1-Ig (same clone as 66305-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 66305-1-Ig (same clone as 66305-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66305-1-Ig (Vinculin Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66305-1-Ig (Vinculin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66305-1-Ig (Vinculin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66305-1-Ig(Vinculin antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66305-1-Ig (same clone as 66305-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vinculin Monoclonal antibody (66305-1-Ig, Clone:2B5A7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66305-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66305-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Vinculin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vinculin fusion protein Ag24946
• Vollständiger Name: vinculin
• Berechnetes Molekulargewicht: 1133 aa, 124 kDa
• Beobachtetes Molekulargewicht: 117 kDa
• GenBank-Zugangsnummer: BC039174
• Gene symbol: Vinculin
• Gene ID (NCBI): 7414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.