Vinculin Monoklonaler Antikörper

Vinculin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3A11F8

Rezensionen (3)

Kat-Nr. : 66305-2-Ig

Synonyme

3A11F8, CMD1W, Metavinculin, MV, MVCL

---

• 

  WB analysis using 66305-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66305-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66305-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 66305-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 66305-2-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 66305-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HGC-27 cells, HeLa-Zellen, HUVEC-Zellen, K-562-Zellen, LNCaP-Zellen, MKN-45-Zellen, Mausherzgewebe, Kaninchenherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66305-2-Ig bindet in WB, IHC, IF/ICC, ELISA Vinculin und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Vinculin fusion protein Ag24946
• Vollständiger Name: vinculin
• Berechnetes Molekulargewicht: 1133 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC039174
• Gene symbol: Vinculin
• Gene ID (NCBI): 7414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Publikationen

Species	Application	Title
	WB	Adv Sci (Weinh); RFWD3 Reprograms Nucleotide Metabolism Through PHGDH to Induce Chemoresistance In Osteosarcoma; Authors - Wenchao Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Matthieu (Verified Customer) (09-24-2025)

Band is very clear at the expected size

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: human fibroblasts

FH

Manon (Verified Customer) (09-24-2025)

Good housekeeping antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Fibroblasts

FH

Emilie (Verified Customer) (07-23-2025)

Used for Western blot on human fibroblasts and gives a clear, well-defined band. I recommend this product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts